SUCCESS: Series of Unique Creative Continuing Education Self Studies

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CEU COURSE: ANTIBODY DETECTION & ANTIBODY IDENTIFICATION

Pre-transfusion testing techniques are described for recipient and donor samples with an emphasis on antibody detection and identification. Additional details are provided related to screening cell dosage, the use of an auto control and the use of probability values in antibody identification.

In this program, the steps taken in the identification of an antibody are presented. These include testing the serum with a panel of reagent red cells with known antigenic determinants through various phases of testing. Once results are obtained, the antibody identification process can begin. This process entails reviewing previous test records, obtaining transfusion and medication histories, excluding all clinically significant antibodies and applying a minimum of the 2+/2- rule to identify and confirm the demonstrating antibody. In most situations, the above-described process completely identifies and confirms the antibody and appropriate antigen-negative red cell products may be selected.

TARGET AUDIENCE: Technologists, Technicians, Managers

LEVEL: Intermediate

SUCCESSTM CREDITS: 1.5 CEUs

Enter Course

LEARNING OBJECTIVES
At the conclusion of this course, participants should be able to:

  1. List the pre-transfusion testing required prior to issuing blood for transfusion.
  2. Describe the antibody screening test and list the types of screening cells available.
  3. Define dosage and give examples of how the screening cell reagents are designed to detect antibodies that exhibit dosage.
  4. Describe the significance of a positive antibody screen in terms of the phases of reactivity observed.
  5. List the reasons for running an auto control during antibody identification.
  6. Describe the 3+/3- and 2+/2- rules in antibody identification.
LEARNING TASKS
To receive CEU credit for this course, participants must:
  1. Review the presentation "Antibody Detection & Antibody Identification" or read the PowerPoint slides and accompanying notes available as a printable Adobe ReaderĀ® file.
  2. Take the online Post-Test.
  3. Complete the online Course Evaluation.
  4. Print the certificate awarding 1.5 SUCCESSTM program CEUs.
FACULTY
Cindy Flickinger, MT(ASCP)SBB
Supervisor, National Reference Laboratory for Blood Group Serology
American Red Cross Biomedical Services, Penn-Jersey Region
Philadelphia, PA

LEARNING REFERENCES
  1. Trudell, K.S. (2005). Chapter 12: Detection and identification of antibodies. In D.M. Harmening (Ed.), Modern Blood Banking & Transfusion Practices, 5thed. (pp. 242 - 263). Philadelphia, PA: F.A. Davis Company.
  2. Walker, P.S. (2008). Chapter 16: Identification of antibodies to red cell antigens. In Technical Manual, 16th ed. (pp. 465 - 498). Bethesda, MD: AABB.
DISCLAIMER
The opinions expressed in this educational activity are those of the faculty and do not necessarily represent the views of the American Red Cross Biomedical Services. Information herein is presented for educational purposes and not to endorse off-label use. Please refer to the official prescribing information for each product for discussion of approved indications, contraindications and warnings.

RELEASE DATE: 10/01/2009

REVIEW DATE: 12/01/2016

EXPIRATION DATE: 11/30/2019

ESTIMATED TIME TO COMPLETE ACTIVITY:
1.5 hours